By Ronald Wetzel, Indu Kheterpal
The facility of polypeptides to shape then again folded, polymeric constructions akin to amyloids and similar aggregates is being more and more famous as a huge new frontier in protein study. This new quantity of Methods in Enzymology besides half B (volume 412) on Amyloid, Prions and different Protein Aggregates proceed within the culture of the 1st quantity (309) in containing certain protocols and methodological insights, supplied by means of leaders within the box, into the newest tools for investigating the constructions, mechanisms of formation, and organic actions of this crucial type of protein assemblies.
- Presents special protocols
- Includes troubleshooting tips
- Provides insurance on structural biology, computational equipment, and biology
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Additional info for Amyloid Prions and Other Protein Aggregates
Coli AXH domain TBP RECOMBINANTLY EXPRESSED His6‐tagged His6‐GST fusion (cleaved) GST‐fusion (cleaved) His6‐tagged MBP‐fusion (intact) Q27, Q78 GST‐fusion (intact) QHQ, Q28, Q50, Q64, Q84 GST‐fusion (cleaved) Q18 Q26, Q36 Ataxin‐1 Huntingtin Purification tags (polyQ length) HAVE BEEN  Whole protein E. coli E. , 2003b E. coli E. coli E. , 2004 E. coli coli coli coli coli E. coli GST‐fusion (cleaved) No tag Q38 E. coli E. coli His6‐tagged Q38 GST‐fusion (cleaved) Q38 GST fusion (cleaved) Q23 His6‐tagged polyQ not stated E.
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Amyloid Prions and Other Protein Aggregates by Ronald Wetzel, Indu Kheterpal