Read e-book online Amyloid Prions and Other Protein Aggregates PDF

By Ronald Wetzel, Indu Kheterpal

The facility of polypeptides to shape then again folded, polymeric constructions akin to amyloids and similar aggregates is being more and more famous as a huge new frontier in protein study. This new quantity of Methods in Enzymology besides half B (volume 412) on Amyloid, Prions and different Protein Aggregates proceed within the culture of the 1st quantity (309) in containing certain protocols and methodological insights, supplied by means of leaders within the box, into the newest tools for investigating the constructions, mechanisms of formation, and organic actions of this crucial type of protein assemblies.

  • Presents special protocols
  • Includes troubleshooting tips
  • Provides insurance on structural biology, computational equipment, and biology

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Coli AXH domain TBP RECOMBINANTLY EXPRESSED His6‐tagged His6‐GST fusion (cleaved) GST‐fusion (cleaved) His6‐tagged MBP‐fusion (intact) Q27, Q78 GST‐fusion (intact) QHQ, Q28, Q50, Q64, Q84 GST‐fusion (cleaved) Q18 Q26, Q36 Ataxin‐1 Huntingtin Purification tags (polyQ length) HAVE BEEN [1] Whole protein E. coli E. , 2003b E. coli E. coli E. , 2004 E. coli coli coli coli coli E. coli GST‐fusion (cleaved) No tag Q38 E. coli E. coli His6‐tagged Q38 GST‐fusion (cleaved) Q38 GST fusion (cleaved) Q23 His6‐tagged polyQ not stated E.

HOLT, MICHAEL L. JOHNSON, AND GARY K. ACKERS VOLUME 381. Oxygen Sensing Edited by CHANDAN K. SEN AND GREGG L. SEMENZA VOLUME 382. Quinones and Quinone Enzymes (Part B) Edited by HELMUT SIES AND LESTER PACKER VOLUME 383. Numerical Computer Methods (Part D) Edited by LUDWIG BRAND AND MICHAEL L. JOHNSON VOLUME 384. Numerical Computer Methods (Part E) Edited by LUDWIG BRAND AND MICHAEL L. JOHNSON VOLUME 385. Imaging in Biological Research (Part A) Edited by P. MICHAEL CONN VOLUME 386. Imaging in Biological Research (Part B) Edited by P.

T. (2001). Recombinant expression and purification of human androgen receptor in a baculovirus system. Biochem. Biophys. Res. Commun. 284, 828–835. , and Wilson, E. M. (1999). Redox‐dependent DNA binding of the purified androgen receptor: Evidence for disulfide‐linked androgen receptor dimers. Biochemistry 38, 9718–9727. , and Wilson, E. M. (2001). Production and purification of histidine‐tagged dihydrotestosterone‐bound full‐length human androgen receptor. Methods Mol. Biol. 176, 67–79. 6 amyloid, prions, and other protein aggregates, part C [1] FIG.

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Amyloid Prions and Other Protein Aggregates by Ronald Wetzel, Indu Kheterpal

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