Download PDF by Ed Harlow and David Lane: Antibodies - A LABORATORY MANUAL

By Ed Harlow and David Lane

ISBN-10: 0879693142

ISBN-13: 9780879693145

Show description

Read Online or Download Antibodies - A LABORATORY MANUAL PDF

Similar molecular biology books

Download PDF by McGraw-Hill Education: BSCS Biology: A Molecular Approach, Student Edition

A accomplished, inquiry-based method of biology BSCS Biology: A Molecular procedure (Blue model) demanding situations talented and honor scholars to imagine scientifically, to combine recommendations, to investigate info, and to discover advanced matters. This research-based software, constructed with investment from the nationwide technology origin, helps an inquiry method of biology.

Weak Links: The Universal Key to the Stability of Networks - download pdf or read online

How can our societies be stabilized in a challenge? Why do we get pleasure from and comprehend Shakespeare? Why are fruitflies uniform? How do omnivorous consuming conduct relief our survival? What makes the Mona Lisa’s smile attractive? How do girls retain our social constructions intact? – may perhaps there in all probability be a unmarried solution to these kinds of questions?

Download PDF by J. David Sweatt: Mechanisms of Memory, Second Edition

Many that paintings at the mobile and molecular procedures of studying and reminiscence are tempted to throw up their palms in frustration and finish that the matter is insoluble. Human studying and reminiscence is probably going the main hugely developed and complicated organic strategy in lifestyles. This e-book represents step one at commencing to prepare the advanced puzzle of the molecular foundation of reminiscence.

New PDF release: The Physiology of Microalgae

This ebook covers the state of the art of microalgae body structure and biochemistry (and the different –omics). It serves as a key reference paintings for these operating with microalgae, even if within the lab, the sphere, or for advertisement purposes. it's aimed toward new entrants into the sphere (i. e. PhD scholars) in addition to skilled practitioners.

Extra info for Antibodies - A LABORATORY MANUAL

Example text

Aligned here in germ-line DNA is a tandem array of the heavy-chain constant region genes: M-chain (lgM), o-chain (lgD), y3 -chain (lgG 3 ) , y 1 -chain (lgG 1 ), y2 b-chain (lgG 2 b), y2 a-chain (lgG 2 a), E-chain (lgE), and a -chain (lgA) (Fig. 10). In essence, these rearrangements move the same antigen binding site onto different antibody classes and subclasses. This process is known as class switching and is one of the characteristic events of the maturation of the antibody response (Chapter 4).

Acad. Sci. 84:8075- 8079. M. G. N. T. R. A. G. Laver. 1987. How antibodies recognize virus proteins. lmmunol. Today 8:323- 326. R. 1987. The ant ibody combining site: Retrospect and prospect. lmmunol. Today 8:44-45 ANTIBODY RESPONSE An antibody response is the culmination of a series of interactions between macrophages, T lymphocytes, and B lymphocytes, all reacting to the presence of a foreign antigen. The end product of this response is the production of large numbers of antibody molecules that bind specifically to the antigen and speed its removal from the animal.

Possible polyclonal binding 3. Possible multivalent binding to secondary reagents 1. Affinity, 106 mol - 1 (weak signal) to 108 mol- 1 (strong signal) 2. Possible bivalent binding 3. Possible multivalent binding to secondary reagents 4. Possible local concentration effects ANTIBODY-ANTIGEN INTERACTIONS 29 AVIDITY Avidity is a measure of the overall stability of the complex between antibodies and antigens. The overall strength of an antibody- antigen interaction is governed by three major factors, the intrinsic affinity of the antibody for the epitope, the valency of the antibody and antigen, and the geometric arrangement of the interacting components.

Download PDF sample

Antibodies - A LABORATORY MANUAL by Ed Harlow and David Lane


by Daniel
4.3

Rated 4.38 of 5 – based on 41 votes