By Ed Harlow and David Lane
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Extra info for Antibodies - A LABORATORY MANUAL
Aligned here in germ-line DNA is a tandem array of the heavy-chain constant region genes: M-chain (lgM), o-chain (lgD), y3 -chain (lgG 3 ) , y 1 -chain (lgG 1 ), y2 b-chain (lgG 2 b), y2 a-chain (lgG 2 a), E-chain (lgE), and a -chain (lgA) (Fig. 10). In essence, these rearrangements move the same antigen binding site onto different antibody classes and subclasses. This process is known as class switching and is one of the characteristic events of the maturation of the antibody response (Chapter 4).
Acad. Sci. 84:8075- 8079. M. G. N. T. R. A. G. Laver. 1987. How antibodies recognize virus proteins. lmmunol. Today 8:323- 326. R. 1987. The ant ibody combining site: Retrospect and prospect. lmmunol. Today 8:44-45 ANTIBODY RESPONSE An antibody response is the culmination of a series of interactions between macrophages, T lymphocytes, and B lymphocytes, all reacting to the presence of a foreign antigen. The end product of this response is the production of large numbers of antibody molecules that bind specifically to the antigen and speed its removal from the animal.
Possible polyclonal binding 3. Possible multivalent binding to secondary reagents 1. Affinity, 106 mol - 1 (weak signal) to 108 mol- 1 (strong signal) 2. Possible bivalent binding 3. Possible multivalent binding to secondary reagents 4. Possible local concentration effects ANTIBODY-ANTIGEN INTERACTIONS 29 AVIDITY Avidity is a measure of the overall stability of the complex between antibodies and antigens. The overall strength of an antibody- antigen interaction is governed by three major factors, the intrinsic affinity of the antibody for the epitope, the valency of the antibody and antigen, and the geometric arrangement of the interacting components.
Antibodies - A LABORATORY MANUAL by Ed Harlow and David Lane