Biophysical, Chemical and Functional Probes of RNA - download pdf or read online

By Daniel Herschlag

ISBN-10: 0123809223

ISBN-13: 9780123809223

This MIE quantity offers laboratory ideas that goal to foretell the constitution of a protein that can have great implications starting from drug layout, to mobile pathways and their dynamics, to viral access into cells. specialist researchers introduce the main complicated applied sciences and strategies in protein constitution and foldingIncludes recommendations on tiling assays.

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Additional info for Biophysical, Chemical and Functional Probes of RNA Structure, Interactions and Folding: Interaction and Folding

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Kieft*,‡ Contents 4 5 6 6 9 12 14 15 16 16 19 20 24 24 1. Introduction 2. 1. 2. 3. 4. Transcription and purification of RNA 3. 1. 2. 3. 4. 5. Weak anion-exchange FPLC Acknowledgments References Abstract Biophysical studies of RNA require concentrated samples that are chemically and structurally homogeneous. Historically, the most widely used methods for preparing these samples involve in vitro transcription, denaturation of the RNA, purification based on size, and subsequent refolding. These methods are useful but are inherently slow and do not guarantee that the RNA is properly folded.

GTPases Regulating Membrane Targeting and Fusion Edited by WILLIAM E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 404. GTPases Regulating Membrane Dynamics Edited by WILLIAM E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 405. Mass Spectrometry: Modified Proteins and Glycoconjugates Edited by A. L. BURLINGAME VOLUME 406. Regulators and Effectors of Small GTPases: Rho Family Edited by WILLIAM E. BALCH, CHANNING J. DER, AND ALAN HALL VOLUME 407. Regulators and Effectors of Small GTPases: Ras Family Edited by WILLIAM E.

RNA Interference Edited by ENGELKE ROSSI VOLUME 393. Circadian Rhythms Edited by MICHAEL W. YOUNG VOLUME 394. Nuclear Magnetic Resonance of Biological Macromolecules (Part C) Edited by THOMAS L. JAMES VOLUME 395. Producing the Biochemical Data (Part B) Edited by ELIZABETH A. ZIMMER AND ERIC H. ROALSON VOLUME 396. Nitric Oxide (Part E) Edited by LESTER PACKER AND ENRIQUE CADENAS VOLUME 397. Environmental Microbiology Edited by JARED R. LEADBETTER VOLUME 398. Ubiquitin and Protein Degradation (Part A) Edited by RAYMOND J.

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Biophysical, Chemical and Functional Probes of RNA Structure, Interactions and Folding: Interaction and Folding by Daniel Herschlag


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