By Daniel Herschlag
This MIE quantity offers laboratory ideas that goal to foretell the constitution of a protein that can have great implications starting from drug layout, to mobile pathways and their dynamics, to viral access into cells. specialist researchers introduce the main complicated applied sciences and strategies in protein constitution and foldingIncludes recommendations on tiling assays.
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Additional info for Biophysical, Chemical and Functional Probes of RNA Structure, Interactions and Folding: Interaction and Folding
Kieft*,‡ Contents 4 5 6 6 9 12 14 15 16 16 19 20 24 24 1. Introduction 2. 1. 2. 3. 4. Transcription and purification of RNA 3. 1. 2. 3. 4. 5. Weak anion-exchange FPLC Acknowledgments References Abstract Biophysical studies of RNA require concentrated samples that are chemically and structurally homogeneous. Historically, the most widely used methods for preparing these samples involve in vitro transcription, denaturation of the RNA, purification based on size, and subsequent refolding. These methods are useful but are inherently slow and do not guarantee that the RNA is properly folded.
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Biophysical, Chemical and Functional Probes of RNA Structure, Interactions and Folding: Interaction and Folding by Daniel Herschlag